Staff Services / Research Facilities / Core Facilities / Histology Core / Histology Methods and Protocols

General protocols, including decalcification, fixation, tissue process, frozen sectioning, H&E staining, immunofluorescence & immunohistochemistry.

The following are general histology protocols and useful resources for additional information. If you have any questions, please contact the Histology Core Specialist.

Histology Protocols

• Fixative Protocols and Recipes (129 kb pdf file)
• Hematoxylin and Eosin (H&E) Staining – Manual Protocol (126 kb pdf file)
• Hematoxylin and Eosin (H&E) Staining – Autostainer (154 kb pdf file)
• Preparing paraffin embedded tissue for staining (121 kb pdf file)
• Immunohistochemistry Protocol (Paraffin) (133 kb pdf file)
• Methods and Techniques for Frozen Tissue Sections (141 kb pdf file)
• Leica TP1020 – Tissue Programming (145 kb pdf file)
• Methods and Protocols for Decalcification of Bone Material (129 kb pdf file)
• Immunofluorescence staining protocol (130 kb pdf file)

Links to useful web sites

• Histonet Listserver for all areas of histology (and its archives)
• IHC World protocols
• Protocols from Cell Signaling
• Abcam protocols