Flow Cytometry Core
Access advanced instruments and expertise for analyzing and sorting cells.
The Flow Cytometry Core Facility provides access to several flow cytometers and a cell sorter available for use by all researchers at the Keenan Research Center for Biomedical Science, we also accept external requests (fees for service apply).
These technologies allow researchers to investigate surface proteins, intracellular proteins, DNA content (cell cycle), cellular proliferation, apoptosis, phagocytosis, microparticle populations, and identification of mixed populations of cells by immunophenotyping (cellular “barcoding”).
Cell sorting further allows you to select specific populations of interest (i.e., GFP positive/negative, a specific cell subset, high or low protein expression, etc.) and sort the cells into tubes for analysis via downstream applications (co-culture, biochemical analysis, etc.).
Importantly the flow cytometry core offers training, as well as consultation services for protocol development, validation and data analysis, to help support your research project.
Equipment
Training
All new users must attend a separate training session for each type of equipment. Please contact the specialist for more information.
Resources
If you are a new user, require access to the flow room, or require refresher training, please contact the Specialist. See below for general RCF Resources.
External Investigator Resources
If you are an external investigator, please fill out the relevant forms and return to the Flow Cytometry Specialist:
Flow Cytometry External Forms
Contact Us
For inquiries, contact Monika Lodyga at Monika.Lodyga@unityhealth.to
Click here to view all RCF specialists.