Molecular Biology Core Equipment

Illumina Next Seq 550 Next Generation Sequencer

• Targeted DNA sequencing
• Whole transcriptome (RNA) sequencing
• Metagenomic sequencing
• ChIP (chromatin IP) sequencing
• Transcriptome sequencing
• Small RNA sequencing
• For more information please contact Pamela Plant
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Covaris Focused Ultrasonicator

• For more information please contact Pamela Plant

The Invitrogen Qubit 4 Fluorometer is designed to quickly and specifically quantitate DNA or RNA used in library prep for NGS

NanoString

• Highly multiplexed gene expression profiling
• No amplification or reverse transcription of mRNA is required as probes read directly off sample mRNA
• Compatible with RNA extracted from FFPE samples
• For more information please contact Pamela Plant
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BioRad QX200 Digital Droplet PCR for gene expression profiling

• Provides absolute quantification of target DNA or RNA molecules
• Reduces bias from amplification efficiency and PCR inhibitors
• Convenient assay design — standard curves are not required
• Optimal for gene expression analysis of small fold changes for low abundance mRNA and miRNA targets
• For more information please contact Pamela Plant
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QuantStudio 7 Real-Time PCR System

• Real Time PCR machine for absolute and relative quantitation of gene expression
• 384 well format
• Newer version of ViiA 7 – same software
• For more information please contact Pamela Plant
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ABI ViiA7

• Blocks available for 384, 96 Fast & TaqMan Low Density Array formats
• Contact Pamela Plant for list of associated reagents
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Eppendorf Electronic Multidispense Pipettes

• For RNA/cDNA use only
• P2-10 and P10-100 available
• Booking calendar in room 553

Captair RNA/DNA Rolling Clean Hood

• Laminar flow hood with UV light
• On metallic rolling cart with locking wheels

Agilent 2100 Bioanalyzer

• Nano, pico and microRNA analysis
• DNA high sensitivity analysis
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Qubit Flurometer

• For more information please contact Pamela Plant

ABI Veriti Thermocycler

• 96 well format
• Book Now

BioRad ChemiDoc Touch CCD Imager for Western Blots

• Supports the acquisition of images from a wide range of gels and blots using a supersensitive camera with a charge-coupled device (CCD)
• Large maximum aperture lens that provides high chemiluminescent sensitivity and wide dynamic range
• Digital images are processed in Image lab software where quantitation of Westerns  is possible with and without Stain free technology
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Li-Cor Odyssey Infrared Imager for Western Blots

• Ability to quantitatively detect two targets in the same sample lane on Western blot
• Infrared fluorophores enable quantitative protein detection
• Wide linear range with sensitivity equal to or better than that of chemiluminescence
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BioTek Synergy NEO Multimode Plate Reader

• Gen 5 software drives the acquisition and analysis of multiple assay types and detection modes on the machine including: absorbance, fluorescence, luminescence, time resolved fluorescence (TRF), fluorescence polarization (FP) and Alpha technology assays
• For more information please contact Pamela Plant
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Eppendorf Mastercycler Gradient Thermocycler:

• Beckman Allegra centrifuge for 96, 384, TLDA plates
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BioRad GelDoc Easy gel documentation system:

• Specialty service offering Lecia CM 3050S Cryostat, ArcturusXT PiXCell Laser capture Microdissection (LCM) System, Leica LCM
• Book Now