Immunofluorescent Staining – Theory and Protocols

Immunofluorescent (IF) staining is a technique that utilizes the specificity of antibodies to their antigen in order to target fluorescent dyes to a specific biomolecule. 

Immunofluorescent staining theory provides a detailed description of the steps involved in IF staining procedures and ways in which these steps can be modified/optimized for your specific experimental design. IF – Full Protocol Example is a detailed IF protocol which includes reagent information. This protocol is a very good starting point to help with the design and optimization of your own procedures. In addition, there are two condensed protocols, Quick protocol paraformaldehyde fix and Quick protocol methanol fix that can also be used in guiding your experimental design.  

Immunostaining Theory

Full Immunostaining Protocol Example

Quick Protocol – Paraformaldehyde Fixation

Quick Protocol – Methanol Fixation