Pre-Clinical Imaging

The pre-clinical imaging facility offers imaging technology for the research community by providing access to in vivo imaging equipment.

Online scheduling is available only to those individuals who have received training on equipment. Review the Imaging guideline on how to receive training. 


Acquisition Systems:

In Vivo Imaging Equipment:

Whole animal photo-acoustic imaging of anesthetized mice. Capable of longitudinal studies done on the same animal.  Allows for the real time measurement of both deoxygenated and oxygenated hemoglobin without the requirement of labels. In addition, dyes (such as ICG) may used and spectrally unmixed from hemoglobin in post processing steps.
""
Heidleberg Spectralis (HRA+OCT)
Simultaneous fundus (retina, optic disc, macula, fovea, posterior pole) imaging and optical coherence tomography. Adapted for use on animals. Imaging Modalities: OCT, infrared, autoflourescence, fluorescein angiography, ICG angiography, red free photography
""

The Newton 7.0 FT500 is capable of both bioluminescence (2D and 3D tomography) as well as fluorescence imaging.

Designed for preclinical in vivo imaging of small animals with a fully automated heated stage and an integrated anesthesia system – allowing for up to 5 mice to be imaged simultaneously. (Also capable of ex vivo and in vitro applications.)

Camera – wide aperture (f/0.7) 16-bit cooled CCD camera

Fluorescence – 8 LED excitation channels, and 8 narrow bandpass emission filters for a wide array of fluorophores (420nm – 780nm).

Image of NEWTON 7.0

Pre-Clinical Imaging Engagement Survey

The Pre-Clinical Imaging Facility is hoping to gather some information about potential equipment usage. The link to the survey can be found here. If you have any questions, please contact our RCF Specialist, Colleen Ding at Colleen.Ding@unityhealth.to. Thank you!


Data Storage

The KRCBS Bioimaging Network Attached Storage (NAS) is a 60 TB capacity data storage unit that is on a shared network with all of the imaging facility computers, i.e. both acquisition and analysis workstation. The NAS was implemented to facilitate data transfer from acquisition computers (i.e. computers connected to microscopes) to the analysis computer workstations without the need for external hard drives. In addition, both RAW and processed data can be temporarily stored on the NAS for ongoing projects. For guidelines on how to access and use the Bioimaging NAS please refer to the following instructions.


Analysis Workstation

All analysis workstations are available for remote access using TeamViewer. Please see instructions here for remote access. Important reminder: you will still need to book the workstations using RFBMS for remote usage. 

Analysis WorkStationRoomFree Software Licensed Software(s)
Ideal For
OSWindows UsernameTeam Viewer ID
FiJi
Imaris Scene
NIS Elements
Zen 3.2 Lite
Other
1552
xxxxView MSOT
MSOT photoacoustic data: Processing, visualization and analysis
Windows 10Workstation 1 User856083966
2552
xxxTscratchZen Black 2012, Zen Blue 2012
LSM700 data: FRAP and colocalization analysis
Windows 7Workstation 2 User307031234
3552
xxImaris 8.0.2 & 9.6.0, NIS Elements AR, Nikon Batch Deconvolution
Multidimesional data from all confocal systems: Deconvolution, 3D visualization, rendering and analysis, colocalization, particle tracking
Windows 10Workstation 3 User423614944
4552
xxxxQupath
LIFA
KUANT
Meta Imaging Series 7.8

Kuant
SD/TIRF data: Particle tracking (Meta Imaging Series 7.8)
Newton animal imaging analysis (Kuant)
Windows 10Workstation 4 User480470169
5778
xxHalo v2.3.2089.23
QuPath
Slide scanning data: Large 2D data set visualization and analysis
Windows 7 AxioScan User1095965369
6552
xMetaXpress
ImageXpress data: High content, low magnification, 2D image analysis
Windows 7 IXM User1657890043